A conditional knockout-rescue system was developed to construct an in-frame deletion strain of Escherichia coli essential genes. The target was flanked with marker genes and FRT (FLP recognition target) sites. and a plasmid containing arabinose-induced FLP recombinase was transformed. After arabinose induction. https://parisnaturalfoodes.shop/product-category/coffeeberry-body-lotion-coconut/